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VAChT overexpression in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory. The mhChR2 is deed to cause larger stationary photocurrents compared to ChR2.

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The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials.

Hemizygous mice are viable and fertile with normal life expectancy, regardless of maternal or paternal inheritance of the transgene. The donating investigator reports that EYFP expression is tg chat by direct fluorescence epifluorescence microscope. Lower EYFP expression is found in cortex, hippocampus, and other brain regions.

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High EYFP fluorescence is also observed in the ventral gray horn in transverse section of the spinal cord. This VAChT overexpression in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory. Compared to wildtype animals, ChAT-ChR2-EYFP line 6 mice show no changes in metabolic profile, locomotor activity in a novel environment, anxiety-like behavior, depression-like behavior, gross sensorimotor function, and ability to use cues to learn a task. Guoping Feng Massachusetts Institute of Technology.

First, a channelrhodopsin-2 cDNA sequence derived from tg chat alga Chlamydomonas reinhardtii was modified to harbor codons optimized for mammalian expression and a gain-of-function HR substitution CAC to CGC deed to cause larger stationary photocurrents.

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This was deed to disable transcription from the Chat locus, and none of the other loci on the BAC were altered. The BAC also includes the vesicular acetylcholine transporter gene Slc18a3 or VAChT that, because it is located in the intron between the first and second exons of the Chat gene, is under the control of the Chat promoter. Upon arrival, sperm was cryopreserved. There are tg chat 50 copies of the transgene present.

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We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Congenic Transgenic. Place Order Live mice available in varying quantities. Ask Customer Service for details. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function.

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Slc18a3 is located in the intron between the first and second exons of the Chat gene and is under the control of the Chat promoter. VAChT protein is overexpressed in the hippocampus and brainstem. EYFP expression is visible by direct fluorescence epifluorescence microscope.

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Disease Terms. The following phenotype relates to a compound genotype created using this strain. Technical Support. Domestic International. Payment Terms tg chat Conditions Terms are granted by individual review and stated on the customer invoice s and statement. Terms Of Use. Questions about Terms of Use. Licensing Information Phone: : TechTran jax. Related Strains.

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Stock No:. Related By:. Did you find what you were looking for? Yes No. How easy was it to find what you were looking for? EYFP expression in cholinergic neurons of the striatum, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei. Slc18a3solute carrier family 18 vesicular monoaminemember 3, mouse, laboratory.

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Guoping Feng, Massachusetts Institute of Technology. AX11 Maximum. Frozen Mouse Embryo.

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